Quercetin Regulates Inflammatory Response of Retinal Ganglion Cells by Affecting the Tumor Necrosis Factor Receptor Associated Factor 6/Transforming Growth Factor-β-Activated Kinase-1 Signaling Pathway

To investigate the protective effect of quercetin on lipopolysaccharide-induced inflammatory response retinal ganglion cell line 5 cells and to explore its possible molecular mechanism. Retinal were cultured in vitro assigned into blank control group, lipopolysaccharide (1.0 μg/ml) induction lipopolysaccharide+low dose (0.25 lipopolysaccharide+medium (0.5 group lipopolysaccharide+high group. The relative survival rate was determined by counting kit-8 assay levels factors interleukin-6 tumor necrosis factor-alpha detected using enzymelinked immunosorbent kits. In addition, factor receptor-associated 6, phosphorylation-transforming growth factor-beta-activated kinase 1 transforming factorbeta- activated measured western blotting. results revealed that could dramatically elevate cells. different groups significantly lower than those supernatant declined with increase concentration a concentration-dependent manner. expression receptorassociated 6 remarkably they also Quercetin displays against mechanism is related suppression activation 6/transforming signaling pathway.